Evaluation of pollination control methods for pigeonpea (Cajanus cajan (L.) Millsp.) germplasm regeneration

Abstract

Maintaining the genetic integrity of germplasm accessions during regeneration is of paramount importance in ex situ conservation of plant genetic resources. In pigeonpea (Cajanus cajan (L.) Millsp.) where outcrossing by insects ranges from 3 to 26% (Reddy et al. 2004), regeneration is costly in terms of time and resources (Remanandan et al. 1988). The problems are compounded when several hundred germplasm accessions need to be regenerated in a season. Nestor and Ramanatha Rao (1998), analyzing the information on seed germplasm regeneration, noted much conjecture and uncertainty over regeneration procedures employed by genebanks. Therefore, the development of optimal procedures for regeneration, to preclude contamination of pollination, is vital to maintain genetic integrity of pigeonpea accessions